rabbit polyclonal anti-e2f5 antibody  (Santa Cruz Biotechnology)

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: (A) Normalized expression of E2F5 in whole mammary glands at different stages of development: virgin, pregnancy day 6 (P6), pregnancy day 18 (P18), lactation day 1 (L1), and lactation day 10 (L10). (B) Normalized expression of E2F5 in basal and luminal cells isolated from mammary glands at different stages: virgin, pregnancy day 18.5 (Preg day 18.5), and lactation day 2 (Lac day 2). (C) Venn diagram showing overlap between E2f5 CUT&RUN target genes and differentially expressed genes in early pregnancy (virgin vs. P6). (D) Normalized expression of several up-regulated E2f5 target genes known to be associated with proliferation and differentiation during pregnancy. (E) Volcano plot of differentially expressed genes between virgin and P6, with E2f5 targets labeled.

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques: Expressing, Isolation, Labeling

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: Paired mammary wholemounts (A) and H&E staining (B) from 12 weeks virgin adult E2F5 CKO, day 5 pregnant E2F5 f/f and day 5 pregnant E2F5 CKO mice shown an impairment on alveoli formation during pregnancy on mice lacking E2F5 in mammary epithelium. (C) PCNA expression in pregnant mice shows reduced proliferative activity in E2F5 CKO mice. (D) Alveoli quantification shown reduced proliferative activity in mice lacking E2F5 in mammary epithelium (p=0.0025).

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques: Staining, Expressing, Activity Assay

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: (A) Early pregnancy in E2F5 CKO females undergoing a second gestation shows reduced number of alveoli in comparison with E2F5 f/f group. Upon day 14 of pregnancy, E2F5 CKO females shows non phenotypic differences in comparison with control groups. (B) Matched histological sections shown evidence of early but no late pregnancy defects in E2F5 CKO females.

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques: Comparison, Control

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: (A) Principal component analysis (PCA) plot showing clustering of CUT&RUN data based on chromatin occupancy for E2F5, H3K4me3, H3K27me3, and IgG across two replicates each. (B) De novo motif discovery using MEME-ChIP revealed a motif that is distinct from the known E2F consensus motif. (C) Motif similarity analysis using Tomtom identified overlap between the discovered E2F5 motif and the known E2F consensus motif. (D) Integrative Genomics Viewer (IGV) tracks showing E2F5 and H3K4me3 occupancy at the promoters of known cell cycle regulatory genes, including CDKN2A, CDKN2B, CDKN2C, CDK4, and CCND1, supporting the role of E2F5 in regulating genes involved in cellular proliferation.

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques:

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: (A) Integrative Genomics Viewer (IGV) tracks showing E2F5, H3K4me3, and IgG CUT&RUN signal at the STAT6 promoter in HC11 mouse mammary epithelial cells. (B) Luciferase reporter assay in 293T cells showing that E2F5 represses STAT6 promoter activity. (C) STAT6 promoter-luciferase reporter assay of MCF7 cells treated with vehicle or R5020 and co-transfected with CMV-HA-E2F5 or CMV-Neo-Bam (empty vector).

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques: Luciferase, Reporter Assay, Activity Assay, Transfection, Plasmid Preparation

Journal: bioRxiv

Article Title: Transcriptional Regulation of Mammary Alveolar Proliferation and Differentiation during Early Pregnancy

doi: 10.1101/2024.11.27.625731

Figure Lengend Snippet: In the absence of progesterone signaling, E2F5 binds to the STAT6 promoter and represses its transcription (left panel). Upon activation of PR, E2F5-mediated repression is relieved, leading to transcriptional activation of STAT6. This could occur either by displacement of E2F5 from the STAT6 promoter or through cooperation between E2F5 and activated PR at the STAT6 promoter (right panel).

Article Snippet: For CUT&RUN, rabbit IgG (Epicypher #13-0042; RRID:AB_2923178), rabbit monoclonal anti-H3K27me3 (Invitrogen #MA5-11198; RRID:AB_1100074), rabbit monoclonal anti-H3K4me3 (Epicypher #13-0041; RRID:AB_3076423), and rabbit polyclonal anti-E2F5 (Invitrogen #PA585578; RRID:AB_2792718) were used at 0.5 ug per sample replicate in 50 uL of volume.

Techniques: Activation Assay

Journal: BMC Cancer

Article Title: E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer

doi: 10.1186/1471-2407-10-64

Figure Lengend Snippet: Clinical characteristics and age distribution of study samples (serum samples) used for E2F5 and CA125 expression studies.

Article Snippet: Primary antibodies directed against the AREB6 (AVIVA Systems Biology, San Diego, California, USA), E2F5 (Acris GmbH, Hiddenhausen, Germany), PAX8 (Abcam Limited, Cambridge, UK), and ELF3 (Orbigen Inc, San Diego, CA) were chosen as the four TFs suggested by the bioinformatics analysis.

Techniques: Expressing

Journal: BMC Cancer

Article Title: E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer

doi: 10.1186/1471-2407-10-64

Figure Lengend Snippet: Summary of Immunohistochemistry results for E2F5 antibody tested on OEC

Article Snippet: Primary antibodies directed against the AREB6 (AVIVA Systems Biology, San Diego, California, USA), E2F5 (Acris GmbH, Hiddenhausen, Germany), PAX8 (Abcam Limited, Cambridge, UK), and ELF3 (Orbigen Inc, San Diego, CA) were chosen as the four TFs suggested by the bioinformatics analysis.

Techniques: Immunohistochemistry

Journal: BMC Cancer

Article Title: E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer

doi: 10.1186/1471-2407-10-64

Figure Lengend Snippet: Validation of potential target (AREB6, PAX8, ELF3) using western blots (A) and expression of E2F5 in serum from normal, benign and, malignant patient samples (B) . (A) Target validation using western blots of AREB6, PAX8, ELF3, in serum of healthy volunteers (N) (n = 2), patients with benign ovarian cysts (B1, B2) (n = 2), and patients with late- (L1, L2) (n = 2) and early-stage (E1-E3) (n = 3) serous adenocarcinoma. AREB6 was overexpressed in late stage disease, but was not discriminatory for early cancer. PAX8 and ELF3 were equally expressed in all serum samples. (B) Expression of E2F5 in serum from normal (n = 56), benign (n = 40) and, malignant (n = 48) serum obtained from patients with cancer of the ovary.

Article Snippet: Primary antibodies directed against the AREB6 (AVIVA Systems Biology, San Diego, California, USA), E2F5 (Acris GmbH, Hiddenhausen, Germany), PAX8 (Abcam Limited, Cambridge, UK), and ELF3 (Orbigen Inc, San Diego, CA) were chosen as the four TFs suggested by the bioinformatics analysis.

Techniques: Biomarker Discovery, Western Blot, Expressing

Journal: BMC Cancer

Article Title: E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer

doi: 10.1186/1471-2407-10-64

Figure Lengend Snippet: Details of E2F5/CA125 expression pattern on different subtypes of OEC performed using western blotting technique.

Article Snippet: Primary antibodies directed against the AREB6 (AVIVA Systems Biology, San Diego, California, USA), E2F5 (Acris GmbH, Hiddenhausen, Germany), PAX8 (Abcam Limited, Cambridge, UK), and ELF3 (Orbigen Inc, San Diego, CA) were chosen as the four TFs suggested by the bioinformatics analysis.

Techniques: Expressing, Western Blot

Journal: BMC Cancer

Article Title: E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer

doi: 10.1186/1471-2407-10-64

Figure Lengend Snippet: Diagnostic accuracy developed for ovarian cancer detection with 13 features using Kstar classification based on SMOTE algorithms.

Article Snippet: Primary antibodies directed against the AREB6 (AVIVA Systems Biology, San Diego, California, USA), E2F5 (Acris GmbH, Hiddenhausen, Germany), PAX8 (Abcam Limited, Cambridge, UK), and ELF3 (Orbigen Inc, San Diego, CA) were chosen as the four TFs suggested by the bioinformatics analysis.

Techniques: Diagnostic Assay